| External factors: | H2O2 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | HDFs were pretreated with C. asiatica extracts or DMSO (control) for 4 h. After pretreatment, premature senescence was induced by 2-h treatment with 200 lm H2O2 and then the cells were transferred to DMEM media containing C. asiatica extracts and cultured for a further 48 h. The percentage of senescent cells increased significantly following treatment with 200 lm H2O2. |
| Regulatory pathway: | p53-p21 |
| R-EF-Pathway: | Upregulation |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Western blot |
| Pathway description: | The results of Western blotting showed that H2O2 induced a robust increase in the expression of p53, p21 and pRb and that pretreatment with C. asiatica extracts inhibited the accumulation of these proteins . |
Annotation: