| External factors: | Bufalin |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Prostate cancer |
| Experiment: | Flow cytometry//SA-β-gal activity assay//Cell morphological analysis |
| Description: | Cell cycle distribution analyses showed increased sub G0/1 apoptotic fraction at bufalin exposure of 20 nM.We examined cellular senescence phenotype in bufalin-exposed LNCaP (PTEN-negative, high AKT activity) cells by evaluating SA-β-gal activity as a cytochemical marker and observed increased proportion of cells with blue staining and flattened morphology. |
| Regulatory pathway: | p53-p21 |
| R-EF-Pathway: | Upregulation |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Western blot |
| Pathway description: | Bufalin treatment for 48 h led to an increased total P53 protein abundance at 10-20 nM as well as that of P21CIP1 in a concentration-dependent manner. |
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