| External factors: | 2,2'-Azobis(2-amidinopropane)dihydrochloride(AAPH) |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//SAHF//Western blot |
| Description: | The results show that AAPH dramatically increased the SA β-Gal activity in A375 and NIH3T3 cells, which was significantly suppressed by NAC.AAPH caused robust SAHF formation in A375 cells, which was significantly reduced by NAC.AAPH significantly increased the protein level of p21 and elevated p53 phosphorylation in A375 cells, indicating activation of this pathway. |
| Regulatory pathway: | p53-p21 |
| R-EF-Pathway: | Activation |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Western blot |
| Pathway description: | AAPH significantly increased the protein level of p21 and elevated p53 phosphorylation in A375 cells, indicating activation of this pathway. |
Annotation: