| External factors: | Cisplatin |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Melanoma |
| Experiment: | SA-β-gal activity assay |
| Description: | 4 days after the CDDP treatment, the β-gal-positive (blue-stained) cells were observed when CDDP was greater than 2 μM. |
| Regulatory pathway: | p53-p21 |
| R-EF-Pathway: | Upregulation |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Western blot//qRT-PCR |
| Pathway description: | The results showed that the increase of γ-H2AX occurred first, followed by DDB2 (a key protein involved in DDR), p-P53 and P53, and then by P21. The upregulation of P21 was maintained up to7 days .Quantitative reverse transcription PCR (qRT–PCR) showed that, CDDP significantly increased the mRNA expression of P21 but not that of P53 or P16. At 7 days after the CDDP treatment, immunofluorescence revealed the obvious accumulation of P21 in the cell nuclei, with the strongest fluorescence located in the huge, malformed nuclei,a typical characteristic of cell senescence. |
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