| External factors: | High glucose |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Type 2 diabetes mellitus |
| Experiment: | SA-β-gal activity assay |
| Description: | Exposure of EPCs with either 25 mM glucose or 200 uM FFA for 3 days significantly increased the percentage of SA-β-gal EPCs compared with control group. Furthermore, EPCs incubation to combined stimuli displayed much higher percentage of senescent cells. |
| Regulatory pathway: | PGC-1α-SIRT1//SIRT1-p53-p21//p38 |
| R-EF-Pathway: | --//Activation//-- |
| Official symbol(s): | PPARGC1A-SIRT1//SIRT1-CDKN1A-TP53//MAPK14 |
| Pathway experiment: | Western blot//qRT-PCR//RT-PCR |
| Pathway description: | EPCs exposed to 48 h of combined stimuli exhibited significant increase in PGC-1α mRNA and protein levels.SIRT1 mRNA and protein levels in EPCs were significantly decreased after 2 days incubation with combined stimuli relative to control cells. SIRT1 mRNA and protein levels in EPCs were significantly decreased after 2 days incubation with combined stimuli relative to control cells. We also found that mRNA and protein levels of P53 and P21 increased significantly when compared to control cells.The effect of high glucose and FFA induced protein expression of PGC-1α were compromised by P38 MAPK inhibitor SB230580 significantly, while ERK MAPK inhibitor U0126 and JNK MAPK inhibitor SP600125 didn t have the same effects . |
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