| External factors: | 17β-Estradiol |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | Co-incubation with 17b-estradiol significantly inhibited the increase in SA-β-gal-positive cells.17b-estradiol dose-dependently increased telomerase activity. |
| Regulatory pathway: | PI3K-Akt |
| R-EF-Pathway: | Activation |
| Official symbol(s): | PIK3CB-AKT |
| Pathway experiment: | Western blot |
| Pathway description: | Interestingly, pretreatment with phosphatidylinositol 3-kinase (PI3-K) blockers,either wortmannin or LY294002, significantly attenuated the increase inhTERT mRNA induced by 17β-estradiol.We examined the effect of 17β-estradiol on Akt activity in EPCs. EPCs were stimulated with several different doses of 17β-estradiol for 1 h and immunoblots were performed with a phosphospecific Akt antibody directed at the Ser473 phosphorylation site. Stimulation with 17β-estradiol led to a dose-dependent phosphorylation of Akt. |
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