External factors: | mTORC1 |
Aging type: | Prevent |
Aging characteristic: |
Category: | Chemical compounds |
Phenotype: | Aging |
Experiment: | SA-β-gal activity assay//Cell morphological analysis//RT-PCR |
Description: | Upon treatment with rapamycin,the percentage of AKT cells positive for SA-β-GAL was significantly reduced. Rapamycin treatment also dramatically reduced AKT-induced effects on cell size, and the SASP. |
Regulatory pathway: | PI3K-Akt |
R-EF-Pathway: | Activation |
Official symbol(s): | PIK3CB-AKT |
Pathway experiment: | SA-β-gal activity assay//Cell morphological analysis//RT-PCR |
Pathway description: | To determine the contribution of enhanced mTORC1 signalling to AKT-induced senescence, BJ-T cells transduced with myr-AKT or control pBABE were treated with the mTORC1-selective inhibitor, rapamycin and markers of senescence analysed. Upon treatment with rapamycin,the percentage of AKT cells positive for SAbGAL was significantly reduced . Rapamycin treatment also dramatically reduced AKT-induced effects on cell size , and the SASP ,indicating that mTORC1 activity is critical for PI3K/AKT-driven senescence. |
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