Aging Overview

External factors

External factors: mTORC1
Aging type: Prevent
Aging characteristic:
Category: Chemical compounds
Phenotype: Aging
Experimental category: L
Tissue type: --
Cell name: BJ-T
PMID: 21909130
Experiment: SA-β-gal activity assay//Cell morphological analysis//RT-PCR
Description: Upon treatment with rapamycin,the percentage of AKT cells positive for SA-β-GAL was significantly reduced. Rapamycin treatment also dramatically reduced AKT-induced effects on cell size, and the SASP.


Regulatory relationship

Regulatory pathway: PI3K-Akt
R-EF-Pathway: Activation
Official symbol(s): PIK3CB-AKT
Pathway experiment: SA-β-gal activity assay//Cell morphological analysis//RT-PCR
Pathway description: To determine the contribution of enhanced mTORC1 signalling to AKT-induced senescence, BJ-T cells transduced with myr-AKT or control pBABE were treated with the mTORC1-selective inhibitor, rapamycin and markers of senescence analysed. Upon treatment with rapamycin,the percentage of AKT cells positive for SAbGAL was significantly reduced . Rapamycin treatment also dramatically reduced AKT-induced effects on cell size , and the SASP ,indicating that mTORC1 activity is critical for PI3K/AKT-driven senescence.


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view