Aging Overview

External factors

External factors: Leptin/NAP-2
Aging type: Accelerate
Aging characteristic:
Category: Chemical compounds
Phenotype: Aging
Experimental category: HL
Tissue type: --
Cell name: MSC
PMID: 25989537
Experiment: SA-β-gal activity assay//Western blot
Description: When cultured with UC‐MSCs, both leptin and NAP‐2 increased the percentage of SA β‐gal–positive cells and up‐regulated expression of p53 and p21 in a dose‐dependent manner. Strikingly, concomitant stimulation of UC‐MSCs with leptin and NAP‐2 further increased the frequency of SA β‐gal–positive cells (38.2?±?1.4% versus 24.3?±?0.6% with leptin stimulation alone and 26.0?±?2.0% with NAP‐2 stimulation alone) and increased expression of p53 and p21 compared with stimulation of cells with leptin or NAP‐2 alone, which suggests that these 2 factors act synergistically to affect MSC senescence.


Regulatory relationship

Regulatory pathway: PI3K-Akt
R-EF-Pathway: Activation
Official symbol(s): PIK3CB-AKT
Pathway experiment: SA-β-gal activity assay//Western blot
Pathway description: We indeed demonstrated a substantial, dose‐dependent elevation of phospho‐Akt expression in UC‐MSCs treated with leptin or NAP‐2 . Consistent with this, the senescence induced by leptin and/or NAP‐2 was almost completely reversed after LY294002 treatment, which was demonstrated by a decreased number of SA β‐gal–positive cells and by diminished levels of p53 and p21.


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view