| External factors: | High glucose |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | We found that the number of SA-β-Gal-positive cells significantly increased in a time-dependent manner, indicating that ARPE-19 might be senescent when treated with 25 mM glucose for 48 h. High glucose also triggered the cell cycle arrest in ARPE-19, and the cells in G0/G1 phase gradually increased from 63.91% to 78.42% after incubation of ARPE-19 with 25 mM high glucose for 48 h. |
| Regulatory pathway: | PI3K-Akt-mTOR |
| R-EF-Pathway: | Activation |
| Official symbol(s): | PI3K-AKT-MTOR |
| Pathway experiment: | Western blot |
| Pathway description: | We found the up-regulated expression of PI3K, p-AKT and p-mTOR in ARPE-19 treated with 25 mM glucose . |
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