| External factors: | CSE |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | Western blot |
| Description: | We performed western blotting of CDKN2A/p16 and CDKN1A/p21 (senescence-associated cyclin dependent kinase inhibitors). Increased CDKN2A and CDKN1A expression levels indicated acceleration of cellular senescence. |
| Regulatory pathway: | PINK1-PARK2 |
| R-EF-Pathway: | -- |
| Official symbol(s): | PINK1-PRKN |
| Pathway experiment: | Knockdown//SA-β-gal activity assay//Immunofluorescence//Western blot |
| Pathway description: | PINK1 knockdown also enhanced CSE-induced mitochondrial ROS production and HBEC senescence . PINK1 knockdown noticeably reduced PARK2-HA levels in the mitochondrial fraction, while increased PARK2-HA levels were observed in the cytosolic fraction in PARK2-HA-transfected HBEC, supporting the notion that PINK1 is responsible for translocation of PARK2 from the cytoplasm to the mitochondria . |
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