| External factors: | Aspirin |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Colorectal cancer |
| Experiment: | SA-β-gal activity assay//MTT assay |
| Description: | Indeed, we found that aspirin induced cellular senescence, with maximum senescence-inducing effects observed at a concentration of 500 M in SW620 cells. We found that aspirin concentration-dependently reduced cell viability in both SW620 human metastatic colorectal carcinoma cells (p53 mutant-type) and HCT116 human primary colorectal carcinoma cells (p53 wild-type), and that it highly inhibited cell proliferation at concentrations greater than 1 mM . |
| Regulatory pathway: | SIRT1-AMPK |
| R-EF-Pathway: | -- |
| Official symbol(s): | SIRT1-PRKAA1 |
| Pathway experiment: | Western blot |
| Pathway description: | Aspirin treatment was found to dose- and time-dependently increase the protein levels of SIRT1, phospho-AMPK (p-AMPK T172), and p-ACC S79. Interestingly, the increase in SIRT1 protein expression was followed by AMPK activation. |
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