| External factors: | Genistein |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | Pretreatment with genistein (1000?nM) inhibited the protein levels of P16 and P21, and decreased the activity of SA-β-gal. |
| Regulatory pathway: | SIRT1-LKB1-AMPK |
| R-EF-Pathway: | Activation |
| Official symbol(s): | SIRT1-LKB1-AMPK |
| Pathway experiment: | SA-β-gal activity assay//Western blot//Knockdown |
| Pathway description: | The effect of genistein on senescence and was restrained by SIRT1 siRNA, LKB1 siRNA, and AMPK siRNA.Ox-LDL ameliorated the phosphorylation of AMPK (Thr-172), and pretreatment with genistein elevated the phosphorylation of AMPK (Thr-172); meanwhile, the effect of genistein was abolished by SIRT1 siRNA or LKB1 siRNA. Compared with SIRT1 siRNA and LKB1 siRNA, control siRNA had no effect. These data ascertained that the effect of genistein could be associated with activating SIRT1/LKB1/AMPK pathway. |
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