| External factors: | Sjp40 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | Western blot |
| Description: | Western blot: To further confirm the role of Sjp40 on cellular senescence, phosphorylation of Rb, which has been viewed as the crucial step in the progression of G1-S phase transition, was measured by Western blot. We observed obvious dephosphorylation of Rb in Sjp40-treated cells. |
| Regulatory pathway: | SKP2-p27 |
| R-EF-Pathway: | -- |
| Official symbol(s): | SKP2 |
| Pathway experiment: | Western blot//SA-β-gal activity assay//Knockdown |
| Pathway description: | Western blot:Since Sjp40 treatment markedly increased the protein level of P27。SA-β-gal activity assay//Knockdown:SA-β-Gal positive percent mediated by Sjp40 in LX-2 cells obviously reduced via knockdown of P27, which confirmed the crucial role of P27 in regulating cellular aging induced by Sjp40。Western blot：SKP2 over-expression reversed P27 protein level induced by Sjp40, indicating that P27 expression induced by Sjp40 is dependent on SKP2 in LX-2 cells.SA-β-gal activity assay：Over-expression of SKP2 rescued the Sjp40-induced senescence analyzed by SA-β-Gal assay. |
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