| External factors: | Chitosan |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | SA-β-gal activity assay:cellCompared to fibroblasts always on TCPS without culturing on chitosan (w/o treatment), cells after chitosan treatment for 3 days significantly decreased the percentage of SA β-gal-positive cells from about 20% to less than 10% cultured on chitosan for 5 DIV increased the percentage of SA β-gal-positive cells. |
| Regulatory pathway: | TGF-β |
| R-EF-Pathway: | Downregulation |
| Official symbol(s): | TGFB1 |
| Pathway experiment: | SA-β-gal activity assay//Western blot |
| Pathway description: | SA-β-gal activity assay:The tendency of cell senescence increased with increasing the TGF-b concentration. Therefore, we assumed chitosan could suppress TGF-β signaling to delay the progress of fibroblast senescence.Western blot:compared to cells cultured on TCPS, only cells treated by chitosan showed significantly lower expression of TGF-b, Smad2/3 and pSmad2/3 . |
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