Aging Overview

External factors

External factors: 1,25(OH)2D3
Aging type: Prevent
Aging characteristic:
Category: Chemical compounds
Phenotype: Osteoporosis
Experimental category: L
Tissue type: --
Cell name: BM-MSC
PMID: 31880094
Experiment: SA-β-gal activity assay//Western blot
Description: Serum calcium and phosphorus levels were increased significantly in exogenous 1,25(OH)2D3-supplemented 18-month-old mice compared with vehicle-treated mice. In addition, BMD, trabecular bone volume, trabecular number and thickness, osteoblast number, MAR, and BFR were increased significantly ,whereas TRAP-positive osteoclastic number, the percentages of β-gal+, p16+, and IL-6+ osteocytes, and the mRNA levels of TNFα,IL-1α, IL-1β, IL-6, Mmp3, and p16 were all significantly decreased in exogenous 1,25(OH)2D3-supplemented 18-month-old mice compared with vehicle-treated mice.


Regulatory relationship

Regulatory pathway: VDR-Ezh2-p16
R-EF-Pathway: --
Official symbol(s): VDR-EZH2-CDKN2A
Pathway experiment: Western blot//qRT-PCR//CHIP//Luciferase reporter assay
Pathway description: We found that 1,25(OH)2D3 increased the mRNA level of Ezh2 and down-regulated p16 and p19 expression at both protein and mRNA levels .The results of a ChIP-PCR assay demonstrated that the VDR could directly bind to the Ezh2 promoter at the predicted binding site. Luciferase reporter assays demonstrated that treatment of 1,25-(OH)2-D3 increased luciferase activity significantly in BM-MSCs transfected with an Ezh2-Luc plasmid, but failed to activate the mutant Luc reporter.


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view