| External factors: | TCDD |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Flow cytometry //Western blot |
| Description: | After treatment with different doses of TCDD for 96 h, and the rate of SA-β-Gal positive astrocytes was significantly elevated in a dose-dependent manner.Flow cytometry showed that there were more astrocytes restricted in the G1 phase harvested from the TCDD-treated group 74.64 ± 2.9%(P <0.01) than the control group 63.49 ± 3.3%, and cell populations in the S phase decreased in the TCDD-treated group 23.39 ± 3.29% (P <0.01) compared with the control group 15.63 ± 2.05%. The protein levels of p16 and p21 were evidently increased in astrocytes treated with different doses of TCDD for 96 h in a dose-dependent manner. |
| Regulatory pathway: | WNT-β-catenin |
| R-EF-Pathway: | Upregulation |
| Official symbol(s): | CTNNBIP1 |
| Pathway experiment: | Western blot |
| Pathway description: | β-catenin was significantly up-regulated by TCDD in a dose-dependent manner, and peaked at 100 nm TCDD exposure. |
Annotation: