Aging Overview

External factors

External factors: ABT-737
Aging type: Accelerate
Aging characteristic:
Category: Chemical compounds
Phenotype: Prostate cancer
Experimental category: HL
Tissue type: --
Cell name: PV-10,22RV1
PMID: 21084274
Experiment: MTT assay//BrdU assay//Colony formation assay
Description: MTT assay: Long-term exposure of PV-10 cells to ABT-737 induced significant growth inhibition and a G1/S cell cycle blockade. Brdu assay//Colony formation assay: a dose-dependent inhibition of colony formation and an inhibition of BrdU incorporation was seen after the addition of ABT-737 to both PV-10 and 22Rv1 cells.


Regulatory relationship

Target gene: AMT
R-EF-Target gene: Activation
Official symbol(s): AMT
Target gene experiment: Western blot//SA-β-gal activity assay
Target gene description: Western blot: In a pool of PV-10 cells ATM levels were decreased by shRNA, we found that a marked reduction in ATM levels prevented ABT-737 from inducing γ-H2AX.SA-β-gal activity assay: ABT-737 treatment of cells with lower levels of ATM protein kinase caused a markedly reduced level of SA-β-Gal and senescence-like morphologic changes .we measured levels of IL-6 and IL-8 mRNA in PV-10-shRNAmir-ATM and PV-10-shRNAmir-control cells. QT-PCR analysis showed that ATM knockdown prevented the ABT-737–mediated induction of IL-6 and IL-8 mRNA.

Regulatory pathway: p53-p21
R-EF-Pathway: Activation
Official symbol(s): TP53-CDKN1A
Pathway experiment: Western blot//SA-β-gal activity assay
Pathway description: Western blot: Both PV-10 and 22Rv1 cells when treated with ABT-737 show increased levels of both wild-type p53 and p21 protein levels. We find that ABT-737 treatment inhibits Cdk2 activity in a dose-dependent fashion in both PV-10 and 22Rv1 cells. Western blot//SA-β-gal activity assay: Western blot analysis confirmed that after ABT-737 treatment overexpression of DN p53 caused a substantial reduction in p21 and inhibited the ability of ABT-737 to induce SA-β-Gal.


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view