| External factors: | Gamma irradiation |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | We observed a dose-dependent decrease in proliferation that leveled around 5Gy.SABG activity was detected in damaged MSC or fibroblasts 9 days following irradiation while no detectable SABG expression was found baseline (0 Gy). This was confirmed by a significant increase in cell size 8 days after irradiation as measured by flow cytometry.Overall, irradiated MSC display all the critical hallmarks associated with the presence of a senescence-associated persistent DDR. |
| Target gene: | PML |
| R-EF-Target gene: | Upregulation |
| Official symbol(s): | PML |
| Target gene experiment: | Immunoflurescence |
| Target gene description: | Immunoflurescence targeting PML revealed a strong increase of the protein in MSC, 10 days after 5Gy irradiation. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: