Aging Overview

External factors

External factors: IRGs
Aging type: Accelerate
Aging characteristic:
Category: Other
Phenotype: Aging
Experimental category: L
Tissue type: --
Cell name: IMR-90
PMID: 26133385
Experiment: Western blot//BrdU assay//Colony formation assay//Cell morphological analysis//SA-β-gal activity assay//SAHF
Description: IRGs induced a stable cell cycle arrest, as determined by a reduction in cyclin A, the phosphorylation status of RB, and 5-bromo-2′-deoxyuridine (BrdU) inco -rporation.The number of colony-forming cells after 2-wk incubation with compound-free medium was strongly reduced if they were pretreated with IRGs.Cells pretreated with the IRGs typically showed an enlarged cellular morphology with increased SA-β-gal activity. p53 and its target p21 were stably upregulated in IRG-treated cells. the levels of HMGA2(a senescence marker) were increased only at the later time point. SAHF formation was also more evident at d9.


Regulatory relationship

Target gene: AURKA//AURKB
R-EF-Target gene: Downregulation//Downregulation
Official symbol(s): AURKA//AURKB
Target gene experiment: Western blot
Target gene description: All five IRGs exhibited a substantial inhibitory effect against AURKA and AURKB, with stronger effects on AURKB.

Regulatory pathway: p53
R-EF-Pathway: Upregulation
Official symbol(s): TP53
Pathway experiment: Western blot
Pathway description: Of interest, we found that the p53-p21 pathway and HMGA2 were up-regulated, particularly at d9 .


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view