| External factors: | IRGs |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | Western blot//BrdU assay//Colony formation assay//Cell morphological analysis//SA-β-gal activity assay//SAHF |
| Description: | IRGs induced a stable cell cycle arrest, as determined by a reduction in cyclin A, the phosphorylation status of RB, and 5-bromo-2′-deoxyuridine (BrdU) inco -rporation.The number of colony-forming cells after 2-wk incubation with compound-free medium was strongly reduced if they were pretreated with IRGs.Cells pretreated with the IRGs typically showed an enlarged cellular morphology with increased SA-β-gal activity. p53 and its target p21 were stably upregulated in IRG-treated cells. the levels of HMGA2(a senescence marker) were increased only at the later time point. SAHF formation was also more evident at d9. |
| Target gene: | AURKA//AURKB |
| R-EF-Target gene: | Downregulation//Downregulation |
| Official symbol(s): | AURKA//AURKB |
| Target gene experiment: | Western blot |
| Target gene description: | All five IRGs exhibited a substantial inhibitory effect against AURKA and AURKB, with stronger effects on AURKB. |
| Regulatory pathway: | p53 |
| R-EF-Pathway: | Upregulation |
| Official symbol(s): | TP53 |
| Pathway experiment: | Western blot |
| Pathway description: | Of interest, we found that the p53-p21 pathway and HMGA2 were up-regulated, particularly at d9 . |
Annotation: