| External factors: | PUVA |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis//ELISA//qRT-PCR |
| Description: | After 2 weeks, PUVA HDFs showed the typical enlarged and flattened senescent-like morphology and displayed de novo expression of SA-β-gal showing an expression fourfold higher than the control cells.Two weeks after PUVA,we observed a relevant decrease in type I pro-collagen expression and a significant increase in the release of MMP-1. |
| Target gene: | PPARγ |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | PPARG |
| Target gene experiment: | Luciferase reporter assay |
| Target gene description: | PUVA exposure decreased luciferase expression of PPARγ at 24 and 48 h. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: