| External factors: | Melatonin |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Systemic lupus erythematosus |
| Experiment: | SA-β-gal activity assay//Western blot//BrdU assay |
| Description: | Senescent cells were decreased in the melatonin-treated group.P53 and P21 were downregulated whereas Cyclin D1, CDK4, and CDK6 were upregulatedwhen cells were cultured with melatonin.Ten μM melatonin increased HUVEC proliferation in SLE medium. |
| Target gene: | RORα |
| R-EF-Target gene: | Activation |
| Official symbol(s): | RORA |
| Target gene experiment: | Knockdown//SA-β-gal activity assay//Western blot//BrdU assay |
| Target gene description: | RORα-knockdown increased the percentage of senescent HUVECs, although cells were treated with melatonin. P53 and P21 were increased whereas Cyclin D1, CDK4, and CDK6 were decreased when RORα was silenced in HUVECs, although cells were protected with melatonin.However, in the RORα-silenced group, HUVEC proliferation was inhibited, although cells were treated with melatonin. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: