| Pathway description: |
Data clearly reveal that nuclear translocation of NF-kB significantly increased in aged rat, whereas old, betaine-fed rats showed lower levels of NF-kB in a dose-dependent manner compared to their old, nonbetaine-fed counterparts.The binding activity of NF-kB was upregulated during aging and that betaine suppressed up-regulation of NF-kB. NF-kB luciferase activity increased by twofold compared to transfected cell exposure of 0.25 lM t-BHP for 6 hours, and betaine showed a dose-dependent ability to decrease NF-kB luciferase activity. We tested the effects of betaine on IKKa/b, ERK1/2, p38,and JNK expression. Results showed that the addition of betaine significantly inhibited phosphorylation of IKKα/β, ERK1/2, p38, and JNK. Moreover, results showed that the addition of betaine significantly inhibited phosphorylation of NIK, MKK1/2, and MKK3/6. Finally, our data suggested that betaine prevents NF-kB nuclear translocation via NIK/IKK and MAPKs . |