| External factors: | BAPTA |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Colony formation assay |
| Description: | BAPTA application impeded H2O2-induced increase of the cell size as well as SA-β-Gal activity, indicating some modulation of the senescence phenotype.BAPTA treatment led to marked increase in the number of proliferating cells compared to H2O2-stimulated cells, indicating that Ca2+ chelation overcame the growth arrest induced by H2O2. |
| Target gene: | DDR |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | DDR1 |
| Target gene experiment: | Flow cytometry//Western?blot |
| Target gene description: | Loading of H2O2-stimulated cells with BAPTA led to a noticeable decrease in the intracellular ROS levels. Indeed, loading with BAPTA dramatically reduced phosphorylation of each DDR participant as compared to H2O2-treated cells over the entire observation period. |
| Regulatory pathway: | p53-p21-Rb |
| R-EF-Pathway: | Downregulation |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Western blot |
| Pathway description: | Accordingly, in H2O2-treated hMESCs, calcium chelation significantly attenuated phosphorylation of p53, prevented enhanced p21 protein expression and elevated the Rb phosphorylation levels long after senescence induction . |
Annotation: