| External factors: | Glucose |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Diabetes |
| Experiment: | SA-β-gal activity assay |
| Description: | As hypothesized, HG caused notable increases in SA β-gal staining, further confirming our previous report of glucose-induced acceleration of EC senescence . |
| Target gene: | SIRT |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | SIRT |
| Target gene experiment: | Western blot//qRT-PCR//ELISA |
| Target gene description: | HG exposure for 48?hr markedly reduced the mRNA expressions of all mitochondrial SIRTs. No such changes were seen following incubation with 25? mmol/L L-glucose (osmotic control). We further measured protein levels of these SIRTs using ELISA. In keeping with mRNA levels, protein levels of SIRTs 3, 4, and 5 were also significantly reduced under HG environments. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: