| External factors: | EtOH |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Osteoporosis |
| Experiment: | SA-β-gal activity assay |
| Description: | In untreated cells, only 13.1 4.6% cells were positive for SA-β-gal staining but, after exposure to EtOH, the percentage of SA-β-gal-positive cells increased to 17.6 6.4% at 10 mM, 36.2 3.9% at 50 mM and 56.9 6.8% at 250 mM. |
| Target gene: | SIRT1 |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | SIRT1 |
| Target gene experiment: | qRT-PCR//Western blot |
| Target gene description: | The mRNA levels of SIRT1 in BM-MSCs decreased upon treatment with EtOH and the protein levels were confirmed by western blot analysis. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: