| External factors: | H2O2 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | The old cells and H2O2-treated cells were higher stained with blue color than young cells. |
| Target gene: | IGFBP3 |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | IGFBP3 |
| Target gene experiment: | Western blot |
| Target gene description: | The expression level of IGFBP-3 also remarkably was decreased in H2O2-induced old cells than young cells. |
| Regulatory pathway: | p53//PI3K-Akt-mTOR |
| R-EF-Pathway: | Activation//-- |
| Official symbol(s): | TP53//PI3K-AKT-MTOR |
| Pathway experiment: | Western blot |
| Pathway description: | The expression levels of p300, Ac-p53, p53, p-p21 and p16 also were increased in H2O2-induced old cells higher than young cells. In contrast, the expression levels of SIRT-1,SIRT-6, MDM2 and p-p53 were increased in H2O2-induced old cells in comparison with young cells;The expression level of IGFBP-3 also remarkably was decreased in H2O2-induced old cells than young cells. In contrast, the expression levels of p-PI3K, Akt-1, p-mTOR, p-FoxO1 and FoxO1 were increased in H2O2-induced old cells in comparison with young cells. |
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