| External factors: | Hyperphosphatemia |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | Western blot//SA-β-gal activity assay |
| Description: | Results showed a significant increased expression in all proteins （p53, p21 and p16）after 24 h of treatment；Senescence was also analyzed, in the same experimental conditions,by the SA-β-gal activity, using a fluorescent probe, by confocal microscopy. A strong fluorescent signal was found after 24 h of treatment which was progressively increasing until 72 h. |
| Target gene: | INTEGRIN LINKED KINASE (ILK) |
| R-EF-Target gene: | Upregulation |
| Official symbol(s): | INTEGRIN LINKED KINASE (ILK) |
| Target gene experiment: | Western blot |
| Target gene description: | ILK protein expression was analyzed in HASMC treated with 10 mM BGP during 24, 48 or 72 h and results showed a significant increase after 24 h of treatment, which remained until 72 h. BGP also increased ILK activity, measured as the GSK-3β phosphorylation. |
| Regulatory pathway: | IGF1-Akt-FoxO |
| R-EF-Pathway: | Activation |
| Official symbol(s): | IGF1-AKT |
| Pathway experiment: | Western blot |
| Pathway description: | We analyzed whether BGP treatment activated the IGF-1 pathway,by evaluating the IGF-1R phosphorylation,and found a sustained increase from 24 to 72h .significant increase in AKT phosphorylation at Ser473 was observed after BGP treatment.Results showed that BGP additionincreased FoxO1 phosphorylation andtotal FoxO1 expression from 24-72h after treatment . |
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