| External factors: | 20(s)-Rg3 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Gallbladder cancer |
| Experiment: | SA-β-gal activity assay//Western blot//Cell morphological analysis |
| Description: | Morphological changes of NOZ and GBC-SD cells could be seen. Both cell lines became enlarged and flattened, had more cytoplasmic vacuoles in a concentration-dependent manner, representing a senescence-like state.The higher the concentration of 20(S)-Rg3, the more SA-β-gal positive cells in both cell lines was observed. Moreover, the protein amounts of p53 and p21Cip1 increased in senescent cells but not the p16 and pRB protein . |
| Target gene: | MDM2 |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | MDM2 |
| Target gene experiment: | Western blot |
| Target gene description: | Western blot further showed that MDM2 protein levels were decreased by 20(S)-Rg3 treatment, in a dose- and time-dependent manner. |
| Regulatory pathway: | p53 |
| R-EF-Pathway: | Activation |
| Official symbol(s): | TP53 |
| Pathway experiment: | Western blot |
| Pathway description: | We next investigated the involvement of the transcription-independent function of p53 using a protein synthesis inhibitor, (CHX), and found the suppressive effect of CHX on p53 protein accumulation was neutralized by 20(S)-Rg3 . |
Annotation: