| External factors: | FL118 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Colorectal cancer |
| Experiment: | SA-β-gal activity assay |
| Description: | Results from SA-β-gal activity staining indicated that 10 nmol/L FL118 treatment induced approximately 92% of SA-β-gal positivity in the surviving cells . |
| Target gene: | MDMX |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | MDMX |
| Target gene experiment: | Western blot |
| Target gene description: | Left plot, FL118 treatment induced expression of human Mdm2 (Hdm2) protein but inhibited expression of human MdmX (HdmX) protein after treatment at 10 nmol/L and 100 nmol/L for 24 hours. |
| Regulatory pathway: | p53 |
| R-EF-Pathway: | Activation |
| Official symbol(s): | TP53 |
| Pathway experiment: | Western blot |
| Pathway description: | p53 accumulation was induced by as low as 10 nmol/L FL118 treatment in WT p53–bearing HCT116 and HCT8 cells. Time course experiment with HCT8 cells indicated that FL118 induced evident p53 accumulation as short as 4 hours after treatment and reaching a peak after 24-hour treatment and sustained at least for 48 hours . |
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