| External factors: | Curcumin |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | Pretreatment with curcumin at 10 and 25 μM? could? significantly? attenuate? the? H2O2-induced HUVECs? senescence? (54.40±3.40% vs. 82.90±2.02%; 51.25±2.74% vs. 82.90±2.02%, p<0.01).Pretreatment of 10 and 25 μM?curcumin?remarkably?decreased?the?ratio of cells at G0/G1 stage (60.02±5.22% vs. 78.12±2.91% and 56.57±4.15% vs. 78.12±2.91%, p<0.05). Accordingly, the ratio of cells in S and G2/M?phases?were?increased?in?curcumin pretreated group compared to the H2O2 group (p<0.05). |
| Target gene: | SIRT1//P21 |
| R-EF-Target gene: | Activation//Downregulation |
| Official symbol(s): | SIRT1//P21 |
| Target gene experiment: | Western blot |
| Target gene description: | 100?μM H2O2 remarkably?decreased?the?expression?of?SIRT1?and?pretreatment with curcumin could increase the expression of SIRT1 in H2O2-treated? HUVECs?. Interestingly, treatment of curcumin alone greatly improved the enzymatic activity of SIRT1 and pretreatment of curcumin could also reverse the change of enzymatic activity of SIRT1 in H2O2-treated? HUVECs?.Meanwhile, pretreatment of 25 μM? curcumin? significantly?decreased the expression of p21 in H2O2-treated?HUVECs?. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: