| External factors: | Hypoxia |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | BrdU assay//SA-β-gal activity assay//Western blot//Cell morphological analysis |
| Description: | Specifically, p7 hMSCs in normoxic culture displayed an enlarged flattened morphology, a characteristic of senescent cells, whereas those at the same passage maintained in hypoxic culture maintained spindle-shaped morphology. At cell passage 7, BrdU was incorporated in less than 1% hMSCs in normoxic culture, whereas BrdU was incorporated in 5.15% hMSCs in hypoxic culture.Hypoxia also reduced the number of p7 hMSCs with positive staining for the activity of senescence-associated b-galactosidase. The ratio of senescence-associated β-galactosidase-positive cells to total cells in hypoxic culture was less than that in normoxic culture . However, when comparing the cells at the same passage number between hypoxic and normoxic culture, the expression levels of p16 mRNA transcripts of p7 hMSCs in hypoxic culture were lower than those of the cells in normoxic culture. |
| Target gene: | MIF |
| R-EF-Target gene: | Upregulation |
| Official symbol(s): | MIF |
| Target gene experiment: | Western blot |
| Target gene description: | Likewise, the expression levels of total MIF were upregulated in hypoxic culture than those in normoxic culture. |
| Regulatory pathway: | Akt |
| R-EF-Pathway: | Activation |
| Official symbol(s): | AKT1 |
| Pathway experiment: | Western blot |
| Pathway description: | The results of western blotting showed that the expression levels of total AKT in hMSCs under the normoxic condition were slightly higher than those under the hypoxic condition, but the levels of pAKT were increased in hypoxic culture compared to those in normoxic culture . |
Annotation: