| External factors: | High Glucose |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Diabetes kidney disease |
| Experiment: | Flow cytometry |
| Description: | The results showed that high glucose led to the hypertrophy of HGMCs and decreased cell number. HG group of HGMCs had cell cycle arrest in G1 phase (P<0.05). |
| Target gene: | MIR-126 |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | MIR-126 |
| Target gene experiment: | Flow cytometry//qRT-PCR |
| Target gene description: | Flow cytometry was used to detect the cell cycle, and the results displayed that miR-126 transfection resulted in a decrease in the ratio of cell arrest in the G1 phase (P<0.01)The miR-126 expression in HG group (5.5±0.8) was significantly lower than that in the NG group. |
| Regulatory pathway: | Telomere-p53-p21-Rb |
| R-EF-Pathway: | --//-- |
| Official symbol(s): | RTEL1-TP53-CDKN1A-RB1 |
| Pathway experiment: | Southern blot//Western blot |
| Pathway description: | The osmotic pressure had no significant effect on the telomere TRF length of cells. The telomere TRF length of HGMCs in NG group and HG group was (8.97±0.35) and (6.77±0.65) kb respectively. As compared with NG group, the telomere TRF length in HG group was significantly shortened (P<0.01).The Western blotting results showed that high glucose-induced expression of p53, p21, and Rb in HGMCs was significantly higher than that in NG group . |
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