| External factors: | Glucose |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//DAPI staining//Western blot//Cell morphological analysis |
| Description: | We discovered that the percentages of SA-βgal positive cells were decreased significantly in the LG group compared with the NG and HG groups.Senescence induced by HG conditions was characterized by pronounced punctuated DNA foci, which were visualized by DAPI staining. Moreover, high glucose leads to cell hypertrophy and nuclear volume increase. The protein levels of p16INK4A (another biomarker of cell senescence) expression in the HG group were significantly higher than in the LG and NG groups. |
| Target gene: | SIRT3 |
| R-EF-Target gene: | Inhibition |
| Official symbol(s): | SIRT3 |
| Target gene experiment: | Western blot |
| Target gene description: | Our experimental data showed that the levels of SIRT3 activity were decreased in the HG group compared with the LG and NG groups. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: