| External factors: | Butylidenephthalide |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Brain tumor |
| Experiment: | Western blot//SA-β-gal activity assay |
| Description: | We found that the senescence-associated proteins Rb, p19, and p16 were upregulated after BP treatment.Indeed, the staining intensity of GBM8401 cells was 30 % on BP 133 μM and 43 % on BP 267 μM. It showed a dose-dependent manner cellular senescence after BP treatments. |
| Target gene: | SKP2 |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | SKP2 |
| Target gene experiment: | Western blot//RT-PCR |
| Target gene description: | In the result, we found expression of Skp2 protein decreased in three GBM cell lines after BP treatment. In addition, GBM 8401, the highest Skp2 expression cell line, decreases in Skp2 protein expression and was dependent on both time and BP concentration.Immunohistochemical analysis indicated that tumor tissues from mice treated with BP showed downregulation of Skp2 expression and upregulation of p21 and p27. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: