Aging Overview

External factors

External factors: DNA damage
Aging type: Accelerate
Aging characteristic:
Category: Other
Phenotype: Aging
Experimental category: HL
Tissue type: --
Cell name: FaDu
PMID: 25543088
Experiment: BrdU assay//SA-β-gal activity assay
Description: Cells were challenged in the absence or presence of 10 μM etoposide for 24 h to induce DNA damage,Brdu assay:As expected, FaDu cells displayed several markers of senescence, such as cell flattening, increase in cellular size, significantly decreased 5-bromodeoxyuridine (BrdU) incorporation.SA-β-gal activity assay:and a positive senescence-associated-β-galactosidase (SA-β-Gal) staining in response to etoposide. 66% of FaDu cells stained positive for SA-β-Gal seven days post-treatment.


Regulatory relationship

Target gene: MYBBP1A
R-EF-Target gene: Downregulation
Official symbol(s): MYBBP1A
Target gene experiment: BrdU assay//SA-β-gal activity assay//Knockdown
Target gene description: Knockdown//Brdu assay: it resulted in a slight but significantly decreased amount of BrdU positive cells. Knockdown//SA-β-gal activity assayand induced the relative number of senescent tumor cells at day 5 post-treatment (2–3 fold) as determined by SA-βgal-positive staining.

Regulatory pathway: PI3K-Akt
R-EF-Pathway: --
Official symbol(s): PIK3CB-AKT
Pathway experiment: Western blot
Pathway description: A strong increase in phosphorylation of AKT (pAKT(Ser473)) was detected in response to etoposide by Western blot analysis, which was consistent with previous observation.


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view