External factors: | DNA damage |
Aging type: | Accelerate |
Aging characteristic: |
Category: | Other |
Phenotype: | Aging |
Experiment: | BrdU assay//SA-β-gal activity assay |
Description: | Cells were challenged in the absence or presence of 10 μM etoposide for 24 h to induce DNA damage,Brdu assay:As expected, FaDu cells displayed several markers of senescence, such as cell flattening, increase in cellular size, significantly decreased 5-bromodeoxyuridine (BrdU) incorporation.SA-β-gal activity assay:and a positive senescence-associated-β-galactosidase (SA-β-Gal) staining in response to etoposide. 66% of FaDu cells stained positive for SA-β-Gal seven days post-treatment. |
Target gene: | MYBBP1A |
R-EF-Target gene: | Downregulation |
Official symbol(s): | MYBBP1A |
Target gene experiment: | BrdU assay//SA-β-gal activity assay//Knockdown |
Target gene description: | Knockdown//Brdu assay: it resulted in a slight but significantly decreased amount of BrdU positive cells. Knockdown//SA-β-gal activity assayand induced the relative number of senescent tumor cells at day 5 post-treatment (2–3 fold) as determined by SA-βgal-positive staining. |
Regulatory pathway: | PI3K-Akt |
R-EF-Pathway: | -- |
Official symbol(s): | PIK3CB-AKT |
Pathway experiment: | Western blot |
Pathway description: | A strong increase in phosphorylation of AKT (pAKT(Ser473)) was detected in response to etoposide by Western blot analysis, which was consistent with previous observation. |
Annotation: