| External factors: | H2O2 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | SA-β-gal activity was significantly increased in Ang II and H2O2 stimulated cells in contrast to the control cells (80.63 ± 2.56 and 79.84 ± 3.07% vs. 5.81 ± 0.62%, P\0.01).we used flow cytometry, the cell cycle was arrested at the G0/G1 phase in both Ang II and H2O2-stimulated groups (87.30 ± 2.59 and 82.00 ± 1.66%) as compared to the control group (52.32 ± 2.15, P\0.01). |
| Target gene: | STAT1 |
| R-EF-Target gene: | Activation |
| Official symbol(s): | STAT1 |
| Target gene experiment: | SA-β-gal activity assay//Western blot |
| Target gene description: | Ang II and H2O2 induced STAT1 activation during HMCs senescence, as manifested by an increase in STAT1 phosphorylation.SA-b-gal-positive cells and the percentage of cells at G0/G1 phase was decreased after STAT1 was knocked down . |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: