| External factors: | Angelica sinensis Polysaccharides |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Acute Leukemia |
| Experiment: | SA-β-gal activity assay//Southern blot |
| Description: | After 40 μg/ml ASP treatment for 48h, the staining positive cells are much more than CD34+CD38? cells absence of ASP performed by SA-β-Gal staining assay.The telomere lengths of AML CD34+CD38? cells chromosome ends have been sharply shortened after ASP treatment presented by Southern Blotting. |
| Target gene: | P16//P21//CYCLIN E//CDK4 |
| R-EF-Target gene: | Upregulation//Upregulation//Downregulation//Downregulation |
| Official symbol(s): | P16//P21//CYCLIN E//CDK4 |
| Target gene experiment: | Western blot |
| Target gene description: | As a result, increased level expression of P16INK4a and P21 but decreased level of CDK4 and CyclinE were observed after 40 μg/ml ASP treatment for 48 h . |
| Regulatory pathway: | p53-p21//p16-pRb |
| R-EF-Pathway: | Upregulation//Upregulation |
| Official symbol(s): | TP53-CDKN1A// |
| Pathway experiment: | qRT-PCR |
| Pathway description: | The expressions of these genes have remarkably been enhanced due to ASP treatment, indicating that p53/p21 and p16INK4a/pRb pathways play an important role in the process of ASP-induced AML CD34+CD38? cells senescence. |
Annotation: