Aging Overview

External factors

External factors: Palbociclib
Aging type: Accelerate
Aging characteristic:
Category: Chemical compounds
Phenotype: Malignant pleural mesothelioma
Experimental category: L
Tissue type: --
Cell name: MPM
PMID: 28704762
Experiment: Cell cycle analysis//Cell morphological analysis//SA-β-gal activity assay//Western blot
Description: Palbociclib induced a significant blockade of the progression from G1 to S phase of the cell cycle in all MPM sensitive cells; Interestingly, treatment with palbociclib induced an accumulation of senescent-like cells, characterized by the production of the Senescent-Associated β-galactosidase enzyme (SA-β-Gal) and by flat and enlarged cell morphology;In addition, the level of cyclin D1 was increased, confirming cyclin D1 induction as a marker associated to cellular senescence.


Regulatory relationship

Target gene: P21
R-EF-Target gene: Upregulation
Official symbol(s): P21
Target gene experiment: Western blot
Target gene description: We observed a great increase in p21 expression,that might be ascribed to the release of p21 gene negative regulation exerted by myc, or to AKT/mTOR signaling activation, which has been shown to initiate p21-dependent senescence in particular conditions.

Regulatory pathway: Akt-mTOR
R-EF-Pathway: Activation
Official symbol(s): AKT1-MTOR
Pathway experiment: Western blot
Pathway description: The additive/synergistic inhibitory effects of the drug combination on cell proliferation were associated with a significant downregulation of p-CDK6, p-Rb, Rb levels, as well as with the inhibition of the AKT/mTOR pathway in both MSTO and ZS-LP cells.


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view