| External factors: | AngⅡ |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Cell morphological analysis |
| Description: | SA-β-gal positive cells were significantly increased in Ang II-stimulated cells, in contrast to the control cells (79.79% ± 5.44%; 7.09% ± 2.48%, p < 0.01).Cell morphology was enlarged and flattened after treatment with Ang II. Flow cytometry was used to characterize the nature of cell-cycle arrest. The cell cycle was arrested at the G0/G1 phase in the Ang II-stimulated group (87.98% ±1.83%), compared with the control cells (54.09% ± 1.22%,p < 0.05). |
| Target gene: | STAT1 |
| R-EF-Target gene: | Activation |
| Official symbol(s): | STAT1 |
| Target gene experiment: | Western blot |
| Target gene description: | Ang II induced STAT1 activation during HMC senescence,as manifested by an increase in STAT1 phosphorylation. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: