| External factors: | F14512 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Cancer |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Cell morphological analysis |
| Description: | Cell cycle analysis revealed that the treatment of P388 cells with F14512 or etoposide resulted in S-phase accumulation at 4–8 h of treatment whereas the proportion of cells in G1 phase decreased.P388 cells positive for SA-β-gal demonstrated enlarged morphology.Using this flow cytometric method for quantitative analysis of senescent cells, we observed that F14512 was more successful in inducing premature senescence of P388 cells than etoposide. |
| Target gene: | SURVIVIN |
| R-EF-Target gene: | Upregulation |
| Official symbol(s): | SURVIVIN |
| Target gene experiment: | Western blot//Immunofluorescence |
| Target gene description: | Immunofluorescence experiments revealed a pronounced increase of survivin level in the nucleus and to a lesser extent in the cytoplasm of F14512 or etoposide treated cells .Exposure of cancer cells to F14512 or etoposide resulted in a striking increase in survivin expression by western blotting. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: