| External factors: | TSY-1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | Cell morphological analysis//SA-β-gal activity assay//Telomerase activity assay |
| Description: | Under the microscope field,we found that the total number of healthy HL60 cells significantly decreased after TSY -1 treatment.After treatment,the dark blue staining became larger and denser,and the percentage of labeling cells also increased in HL60 cells.With the treatment of TSY -1, the Telomerase activity decreased significantly in HL60 cells. |
| Target gene: | TERT |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | TERT |
| Target gene experiment: | Western blot |
| Target gene description: | Western blot combined with immunoprecipitation showed that TERT protein level in HL60 cells decreased significantly after TSY-1 treatment by a dose-dependent manner . |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: