| External factors: | The BET bromodomain inhibitor JQ1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Chondrosarcoma |
| Experiment: | Flow cytometry//BrdU assay |
| Description: | Treatment of JQ1 significantly induced G0/G1 cell cycle arrest, and retarded S phase entering,consistent with the results from EdU incorporation assay.We investigated whether JQ1 induces senescence of SW 1353 cells.JQ1 did significantly increase senescence of SW 1353 cells. |
| Target gene: | P21 |
| R-EF-Target gene: | Upregulation |
| Official symbol(s): | P21 |
| Target gene experiment: | Western blot |
| Target gene description: | Knockdown of p21 induced by JQ1 decreased the expression of c-Myc and Bcl-xL, indicating that p21 is indeed an upstream regulator of c-Myc/Bcl-xL axis in the presence of JQ1. |
| Regulatory pathway: | YAP-p21-c-Myc |
| R-EF-Pathway: | Activation |
| Official symbol(s): | YAP-CDKN1A-MYC |
| Pathway experiment: | Western blot//Knockdown |
| Pathway description: | Consistent with this, JQ1 did significantly attenuate the c-Myc expression in both SW 1353 and Hs 819.T cells, as evidence by immunoblotting, suggesting that c-Myc is a direct target of JQ1 in these cells.Knockdown of p21 induced by JQ1 decreased the expression of c-Myc and Bcl-xL, indicating that p21 is indeed an upstream regulator of c-Myc/Bcl-xL axis in the presence of JQ1.We also demonstrated that knockdown of YAP up-regulated the expression of p21,suggesting that p21 is a downstream target of YAP in chondrosarcoma SW 1353 cells. |
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