| External factors: | Matrine |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Immunofluorescence staining//Flow cytometry |
| Description: | Flow cytometric analysis revealed that a greater percentage of U251, U87, and P3 cells were arrested in G1/G0 (~ 60% vs ~ 80% and 100%, control vs U251, U87, and P3 under 0.2 mmol/L matrine for 3 days;Matrine treatment for 3 days also led to increased γH2AX‐positive nuclei indicative of DNA double‐strand breaks , and accumulation of SA‐β‐gal‐positive cells . |
| Target gene: | P27 |
| R-EF-Target gene: | Upregulation |
| Official symbol(s): | P27 |
| Target gene experiment: | Western blot |
| Target gene description: | Matrine treatment induced a marked increase in p27 protein expression in both U251 and U87 cells within 24 hours. |
| Regulatory pathway: | IGF1-PI3K-Akt-p27 |
| R-EF-Pathway: | Downregulation |
| Official symbol(s): | IGF1-PI3K-AKT |
| Pathway experiment: | Western blot//SA-β-gal activity assay |
| Pathway description: | Matrine treatment led to decreased expression of two proteins associated with cycling cells, PI3K and pAKT . Activation of the pathway was partially restored when cells were treated simultaneously with SC79; pAKT was partially increased while p27 was decreased to control levels. exogenous IGF1 partially reversed the expression of PI3K, pAKT, and p27 .Anti‐IGF1 with matrine was more effective at inducing cellular senescence in both U251 and U87 cells than treatment with matrine alone . |
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