| External factors: | Cigarette smoke |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Chronic obstructive pulmonary disease |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | As expected, we found that CSE (0.5%) treatment for both 15 days and 30 days induced cellular senescence, which is characterized by increased senescence-associated β-galactosidase (SA-β-gal) activity, p16 (CDKN2A or INK4A) and p21 (CDKN1A) protein abundance. |
| Target gene: | TPP1//SIRT1 |
| R-EF-Target gene: | Downregulation//-- |
| Official symbol(s): | TPP1//SIRT1 |
| Target gene experiment: | Western blot//Immunofluorescence |
| Target gene description: | Nuclear abundance of TPP1 and TIN2 was reduced with CSE .We performed coimmunoprecipitation of TPP1 with or without CSE (0.5% for 15 days) in HFL1 cells, and probed with acetyl lysine and the Sirt1 antibody. Similarly, Sirt1 pull-down was performed and the lysate was probed for the TPP1 antibody. This was further confirmed by co-immunofluorescence for Sirt1 and TPP1 in HFL1 cells. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: