| External factors: | G-quadruplex ligand 12459 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | Western blot//Immunostaining |
| Description: | Prolongation of 12459 treatment to 8 days provokes a cell growth arrest where P21 expression became readily detectable, suggesting the onset of cell senescence. Results showed that DNA damage foci induced by 12459 co-localized with TRF1 and corresponded to TIFs (Telomere-dysfunction Induced Foci). |
| Target gene: | P53//CHK1 |
| R-EF-Target gene: | Upregulation//Activation |
| Official symbol(s): | P53//CHK1 |
| Target gene experiment: | Western blot |
| Target gene description: | Results indicate that 12459 treatment of A549 cells(0.5–2mM) induces a transient overexpression of p53 after 4 days.In contrast, a significant activation of Chk1(Ser317) was found at the onset of senescence after 8 days that coincides with the expression of p21. |
| Regulatory pathway: | PPM1D-WIP |
| R-EF-Pathway: | Activation |
| Official symbol(s): | PPM1D-WIPF1 |
| Pathway experiment: | Western blot |
| Pathway description: | The expression of PPM1D/WIP1 was thus analyzed by western blot during the time-course induction of p53 by 12459 . Results show that PPM1D/WIP1 expression is activated by 12459 treatment and starts after 2 h. An increased level of PPM1D/WIP1 is maintained up to 48h. |
Annotation: