| External factors: | BTM-0512 |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | A trend for increased senescence, although non-significant, could be observed at 24 h after treating the endothelial cells with D-glucose (30 mmol ? L), which reached the significant level at 48 h after the treatment. The high glucose-induced senescence was significantly reduced in the presence of BTM-0512 at a concentration of more than 3 lmol ? L. L-glucose (30 mmol ? L) and the vehicle (0.1% alcohol) had no effect on cell senescence. |
| Target gene: | SIRT1 |
| R-EF-Target gene: | Activation |
| Official symbol(s): | SIRT1 |
| Target gene experiment: | RT-PCR |
| Target gene description: | Moreover, BTM-0512 also reversed the high glucose-induced downregulation of SIRT1 mRNA expression. |
| Regulatory pathway: | DDAH-ADMA |
| R-EF-Pathway: | -- |
| Official symbol(s): | DDAH1 |
| Pathway experiment: | qPCR//Western blot |
| Pathway description: | Administration of BTM-0512 alone dramatically upregulated DDAH2 mRNA expression and DDAH activity .Transfection of HUVEC with PGCsi-DDAH2-shRNA successfully knocked down the DDAH2 mRNA expression, reduced the DDAH activity and increased the level of ADMA in the cultured medium . |
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