| External factors: | Harmine |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Lung cancer |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | Similar to silencing of TWIST1, treatment of the KRAS mutant NSCLC lines A549, H460, and H358 with harmine-induced changes characteristic of OIS, including positive Senescence-Associated β-galactosidase (SA-β-Gal) staining and induction of p21 and p27. We also found that harmine treatment induced OIS in NSCLC cell lines with EGFR and MET mutations . |
| Target gene: | TWIST1//E3A |
| R-EF-Target gene: | Downregulation//Downregulation |
| Official symbol(s): | TWIST1//E3A |
| Target gene experiment: | Western blot |
| Target gene description: | Harmine treatment reduced the levels of TWIST1 protein in a dose- and time-dependent manner as shown by Western blotting. Remarkably, treatment with harmine resulted in a dose-dependent decrease of E2A protein expression in both KRAS-mutant and MET-mutant/amplified NSCLC cell lines. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: