| External factors: | Sulforaphane |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | There was also a decrease in net L-lactate formation and onset of increased cell senescence - as indicated by decreased cell staining for β-galactosidase activity with SFN treatment. |
| Target gene: | TXNIP//HK2 |
| R-EF-Target gene: | Upregulation//Downregulation |
| Official symbol(s): | TXNIP//HK2 |
| Target gene experiment: | RT-PCR//Western blot |
| Target gene description: | The most profound effect of senescence on the gene expression of MRC-5 cells was the 8-fold increase in the expression of TXNIP. This was increased further to 21- fold by treatment with SFN .HK1 and HK2 mRNA levels were increased in senescent MRC-5 fibroblasts, and this was not corrected by SFN, although there was a time treatment effect where SFN decreased mRNA of HK1 and HK2 in senescence. In BJ fibroblasts, SFN treatment increased HK1 mRNA modestly in both early and late passages whereas for HK2 it was decreased in senescence. A similar effect was found in BJ fibroblasts where SFN decreased HK2 protein in senescence . |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: