| External factors: | 4-hydroxynonenal |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | Treatment with 10 lM 4-HNE increased the proportion of senescent cells, demonstrated by cellular enlargement and positive staining for SA-β-gal . |
| Target gene: | TXNIP//PPARD |
| R-EF-Target gene: | Upregulation//Activation |
| Official symbol(s): | TXNIP//PPARD |
| Target gene experiment: | Western blot |
| Target gene description: | We found increased TXNIP expression in VEC incubated with 10 lM 4-HNE for 7 days.TXNIP expression was increased in VEC co-cultured with foam-cells. The 4-HNE scavenger FL926-A16 decreased TXNIP expression in VEC co-cultured with foam cells.The selective PPARd antagonist GSK0660 abolished 4-HNE effects on the expression of the different senescence markers and prevented the upregulation of TXNIP. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: