| External factors: | Vitamin D |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | Flow cytometry//Western blot//SA-β-gal activity assay |
| Description: | In quiescent HUVEC, 24 h of Vit.D pre-treatment reduced ROS generation induced by increasing doses of IR (p < 0.05), achieving the maximum efficacy at 75 nmol/l. Vit.D at this concentration reduced the ROS production by 33.3 % (RT 2 Gy), 46.1 % (RT 4 Gy), 63 % (RT 6 Gy), 54.1 % (RT 8 Gy). On quiescent HUVEC treated with IR, Vit.D pre-treatment, compared to non-Vit.D pre-treatment, reduced by 46.3 % and increased by 65 % the cells in G0 or G1cell cycle phase, respectively, reduced over 45.7 % the β-galactosidase content compared to cells non Vit.D pretreated and prevented the p53 phosphorylation and both p21Cip1/Waf1 and p16INK4a proteins up-regulation, all marker of cellular senescence. |
| Target gene: | SIRT1 |
| R-EF-Target gene: | Upregulation |
| Official symbol(s): | SIRT1 |
| Target gene experiment: | Western blot |
| Target gene description: | In proliferating HUVEC, Vit.D pre-treatment counteracted SirT1 protein expression downregulation mediated by IR and this action was annulled by U0126-mediated MEKs/ERKs inhibition. |
| Regulatory pathway: | p38 |
| R-EF-Pathway: | Downregulation |
| Official symbol(s): | MAPK14 |
| Pathway experiment: | Western blot//MTT assay//TUNEL assay |
| Pathway description: | MKK6 over-expression activated basal levels of p38 that were further increased by RT and slightly reduced by Vit.D treatment. |
Annotation: